Learn More About The CD44 Antibody

The CD44 antibody is a cell-surface glycoprotein that is expressed on monocytes, lymphocytes, and granulocytes. It is also considered a homing cell adhesion molecule, Hermes-1, HUTCH-1, ECM-III, or a Phagocytic glycoprotein. Likewise, it has been implicated in lymphocyte homing, tumor metastasis, and cell migration.

More Information

The CD44 antibody is to be used for research only. It does have a clone called SPM 521, and the immunogen is the synthetic peptide that corresponds to the human CD44 antibody. The isotype is the IgG2a, and it has an undetermined epitope. Its molecular weight is 80 kDa, and it has been tested in humans. The pre-diluted format comes as 7.0ml, while the concentrated versions offer sizes of 1.0ml, 0.5 ml, and 0.1ml.

Applications

These antibodies can be used with Western Blotting and Immunohistochemistry (IHC) applications. The procedure for IHC includes preparing specimens using paraffin-embedded or Formalin-fixed tissues. Deparaffinized slides must be used along with graded alcohols or xylene. When using the concentrated version, the product must be diluted using a ratio of one to 50. However, there is a pre-diluted version available, as well. To retrieve the antigen, you’ll need to boil the section of tissue using a 10mM citrate buffer with a pH of 6.0 for at least 10 minutes. Then, you should allow it to cool to room temperature for 20 minutes. Incubation periods are 30 minutes at room temperature. The positive control is the esophageal carcinoma.

For Western Blotting techniques, you should dilute the product using a ratio of one to 25. Incubation periods are at least one hour at room temperature. The positive control for this application is the HeLa cell lysate with a cellular localization occurring in the membrane.

The CD44 antibody is an excellent product to help with laboratory research. Visit Spring Bioscience today to find out more and options for purchasing.


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Author: Myrtice Lovett

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